A horizontal frame from 3-D reconstructed three-photon microscopy images in a mouse brain. The red is neurons, the blue is blood vessels, and the dark holes are non-fluorescent neurons. Credit: Xu lab

Three-photon microscopy improves biological imaging

January 22, 2013 by Anne Ju

(Phys.org)—Scientists may be a step closer to cracking one of the world’s most compelling mysteries: the impossible complexity of the brain and its billions of neurons. Cornell researchers have demonstrated a new way of taking high-resolution, three-dimensional images of the brain’s inner workings through a three-fold improvement in the depth limits of multiphoton microscopy, a fluorescence-based imaging technique with Cornell roots.

Publishing in the journal Nature Photonics Jan. 20, senior author Chris Xu, associate professor of applied and engineering physics, and colleagues have demonstrated high-resolution, 3-D imaging of the subcortical region of a live, intact mouse brain.

Read more: Three-photon microscopy improves biological imaging — phys.org.

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